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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
07/12/2015 |
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Data da última atualização: |
26/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, A. S. A. M. T.; LEDUR, M. C.; BOSCHIERO, C.; NONES, K.; PINTO, L. F. B.; JAENISCH, F. R. F.; BURT, D. W.; COUTINHO, L. L. |
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Afiliação: |
ANA SILVIA ALVES MEIRA TAVARES MOURA, UNESP/FMVZ; MONICA CORREA LEDUR, CNPSA; CLARISSA BOSCHIERO, ESALQ; KATIA NONES, QIMR Berghofer Medical Research Institute; LUÍS FERNANDO BATISTA PINTO, UFB/Departamento de Zootecnia; FATIMA REGINA FERREIRA JAENISCH, CNPSA; DAVID W. BURT, University of Edinburgh; LUIZ LEHMANN COUTINHO, ESALQ. |
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Título: |
Quantitative trait loci with sex-specific effects for internal organs weights and hematocrit value in a broiler-layer cross. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Applied Genetics, v. 57, n. 2, p. 215–224, 2015. |
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DOI: |
10.1007/s13353-015-0325-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Rapid growth in broilers is associated with susceptibility to metabolic disorders such as pulmonary hypertension syndrome (ascites) and sudden death. This study describes a genome search for QTL associated with relative weight of cardio respiratory and metabolically important organs (heart, lungs, liver and gizzard), and hematocrit value in a Brazilian broiler-layer cross. QTL with similar or different effects across sexes were investigated. At 42 days of age after fasted for 6 h, the F2 chickens were weighed and slaughtered. Weights and percentages of the weight relative to BW42 of gizzard, heart, lungs, liver and hematocrit were used in the QTL search. Parental, F1 and F2 individuals were genotyped with 128 genetic markers (127 microsatellites and 1 SNP) covering 22 linkage groups. QTL mapping analyses were carried out using mixed models. A total of 11 genome-wide significant QTL and five suggestive linkages were mapped. Thus, genomewide significant QTL with similar effects across sexes were mapped to GGA2, 4 and 14 for heart weight, and to GGA2, 8 and 12 for gizzard %. Additionally, five genome-wide significant QTL with different effects across sexes were mapped to GGA 8, 19 and 26 for heart weight; GGA26 for heart % and GGA3 for hematocrit value. Five QTL were detected in chromosomal regions where QTL for similar traits were previously mapped in other F2 chicken populations. Seven novel genome-wide significant QTL are reported here, and 21 positional candidate genes in QTL regions were identified. MenosAbstract Rapid growth in broilers is associated with susceptibility to metabolic disorders such as pulmonary hypertension syndrome (ascites) and sudden death. This study describes a genome search for QTL associated with relative weight of cardio respiratory and metabolically important organs (heart, lungs, liver and gizzard), and hematocrit value in a Brazilian broiler-layer cross. QTL with similar or different effects across sexes were investigated. At 42 days of age after fasted for 6 h, the F2 chickens were weighed and slaughtered. Weights and percentages of the weight relative to BW42 of gizzard, heart, lungs, liver and hematocrit were used in the QTL search. Parental, F1 and F2 individuals were genotyped with 128 genetic markers (127 microsatellites and 1 SNP) covering 22 linkage groups. QTL mapping analyses were carried out using mixed models. A total of 11 genome-wide significant QTL and five suggestive linkages were mapped. Thus, genomewide significant QTL with similar effects across sexes were mapped to GGA2, 4 and 14 for heart weight, and to GGA2, 8 and 12 for gizzard %. Additionally, five genome-wide significant QTL with different effects across sexes were mapped to GGA 8, 19 and 26 for heart weight; GGA26 for heart % and GGA3 for hematocrit value. Five QTL were detected in chromosomal regions where QTL for similar traits were previously mapped in other F2 chicken populations. Seven novel genome-wide significant QTL are reported here, and 21 positional candidate g... Mostrar Tudo |
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Thesagro: |
Frango de corte; Melhoramento genético animal. |
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Thesaurus Nal: |
Animal genetics; Broiler chickens; Genetic markers; Molecular genetics. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134959/1/final8050.pdf
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Marc: |
LEADER 02444naa a2200289 a 4500
001 2030756
005 2018-01-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s13353-015-0325-2$2DOI
100 1 $aMOURA, A. S. A. M. T.
245 $aQuantitative trait loci with sex-specific effects for internal organs weights and hematocrit value in a broiler-layer cross.$h[electronic resource]
260 $c2015
520 $aAbstract Rapid growth in broilers is associated with susceptibility to metabolic disorders such as pulmonary hypertension syndrome (ascites) and sudden death. This study describes a genome search for QTL associated with relative weight of cardio respiratory and metabolically important organs (heart, lungs, liver and gizzard), and hematocrit value in a Brazilian broiler-layer cross. QTL with similar or different effects across sexes were investigated. At 42 days of age after fasted for 6 h, the F2 chickens were weighed and slaughtered. Weights and percentages of the weight relative to BW42 of gizzard, heart, lungs, liver and hematocrit were used in the QTL search. Parental, F1 and F2 individuals were genotyped with 128 genetic markers (127 microsatellites and 1 SNP) covering 22 linkage groups. QTL mapping analyses were carried out using mixed models. A total of 11 genome-wide significant QTL and five suggestive linkages were mapped. Thus, genomewide significant QTL with similar effects across sexes were mapped to GGA2, 4 and 14 for heart weight, and to GGA2, 8 and 12 for gizzard %. Additionally, five genome-wide significant QTL with different effects across sexes were mapped to GGA 8, 19 and 26 for heart weight; GGA26 for heart % and GGA3 for hematocrit value. Five QTL were detected in chromosomal regions where QTL for similar traits were previously mapped in other F2 chicken populations. Seven novel genome-wide significant QTL are reported here, and 21 positional candidate genes in QTL regions were identified.
650 $aAnimal genetics
650 $aBroiler chickens
650 $aGenetic markers
650 $aMolecular genetics
650 $aFrango de corte
650 $aMelhoramento genético animal
700 1 $aLEDUR, M. C.
700 1 $aBOSCHIERO, C.
700 1 $aNONES, K.
700 1 $aPINTO, L. F. B.
700 1 $aJAENISCH, F. R. F.
700 1 $aBURT, D. W.
700 1 $aCOUTINHO, L. L.
773 $tJournal of Applied Genetics$gv. 57, n. 2, p. 215–224, 2015.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
30/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BIGI, M.; VAZQUEZ, C. L.; CASTELÃO, A. B. C.; GARCÍA, E. A.; CATALDI, A. A.; JACKSON, M.; MAcNEIL, M.; SORIA, M.; ZUMÁRRAGA, M. J.; MATIAS, C.; GAGO, G.; BLANCO, F. C.; NISHIBEF, C.; ALMEIDA, N. F.; ARAUJO, F. R.; BIGI, F. |
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Afiliação: |
Mercedes Bigi, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Cristina Lourdes Vazquez, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Ana Beatriz C Castelão, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Elizabeth Andrea García, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Angel A Cataldi, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Mary Jackson, Colorado State University, Dept. of Microbiology, Immunology and Pathology; Michael McNeil, Colorado State University, Dept. of Microbiology, Immunology and Pathology; Marcelo Soria, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Martín J Zumárraga, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Matias Cabruja, Laboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Gabriela Gago, Laboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Federico C Blanco, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Christiane Nishibef, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Computação; Nalvo F Almeida, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Computação; FLABIO RIBEIRO DE ARAUJO, CNPGC; Fabiana Bigi, Instituto de Biotecnología, IABIMO, CICVyA/INTA. |
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Título: |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 239, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines. MenosMycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. b... Mostrar Tudo |
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Palavras-Chave: |
Mutations. |
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Thesagro: |
Gene. |
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Thesaurus NAL: |
Genome; Mycobacterium bovis BCG; Virulence. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207981/1/Analysing-nonsynonymous-mutations.pdf
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Marc: |
LEADER 02585naa a2200361 a 4500
001 2117921
005 2019-12-30
008 2019 bl uuuu u00u1 u #d
100 1 $aBIGI, M.
245 $aAnalysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles.$h[electronic resource]
260 $c2019
520 $aMycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.
650 $aGenome
650 $aMycobacterium bovis BCG
650 $aVirulence
650 $aGene
653 $aMutations
700 1 $aVAZQUEZ, C. L.
700 1 $aCASTELÃO, A. B. C.
700 1 $aGARCÍA, E. A.
700 1 $aCATALDI, A. A.
700 1 $aJACKSON, M.
700 1 $aMAcNEIL, M.
700 1 $aSORIA, M.
700 1 $aZUMÁRRAGA, M. J.
700 1 $aMATIAS, C.
700 1 $aGAGO, G.
700 1 $aBLANCO, F. C.
700 1 $aNISHIBEF, C.
700 1 $aALMEIDA, N. F.
700 1 $aARAUJO, F. R.
700 1 $aBIGI, F.
773 $tVeterinary Microbiology$gv. 239, 2019.
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